Advances in Chromatography

This book investigates current trends in chemometrics, proteomics, column technology, and element-selective detection for pharmaceutical, medical, industrial, and environmental applications. It offers recent strategies to evaluate and assess materials in air, water, soil, and landfill samples, to determine the amount of hydrocarbons in the atmosphe

Phyllis R Brown, Eli Grushka

1. Chemometric Analysis of Comprehensive Two- Dimensional Separations Robert E. Synovec, Bryan J. Prazen, Kevin J. Johnson, Carlos G. Fraga, and Carsten A. Bruckner I. Introduction II. Comprehensive 2-D Separations III. Chemometric Analysis of Chemical Separation Data IV. Comprehensive 2-D Separations/Gram Analysis V. Feature Selection and Pattern Recognition VI. Scope and Future Directions References 2. Column Technology for Capillary Electrochromatography Luis A. Colon, Todd D. Maloney, Jason Anspach, and Hector Colon I. Introduction II. Open Tubular Columns, III. Packed Columns IV. Monolithic Columns V. Microfabricated Devices VI. Final Remark References 3. Gas Chromatography with Inductively Coupled Plasma Mass Spectrometric Detection (GP-ICP MS) 107 Brice Bouyssiere, Joanna Szpunar, Gaetne Lespes, and Ryszard Lobinski I. Introduction II. Feasibility of an Analysis by GC-ICP MS III. Sample Preparation for GC-ICP MS IV. Interface Between GC and ICP MS V. Low-Power ICP as a Tunable Ionization Source for GC Effluents VI. Mass Spectrometric Detection in Gas Chromatography VII. GC-ICP MS Studies Using Stable Isotopes VIII. Selected Applications References 4. GC-MS Analysis of Halocarbons in the Environment Filippo Mangani, Michela Maione, and Pierangela Palma I. Introduction II. The Analytical Problem References 5. Microfluidics for Ultrasmall-Volume Biological Analysis Todd O. Windman, Barb J. Wyatt, and Mark A. Hayes I. Introduction II. Microscopy, III. Direct Probes IV. Microsampling V. Direct Sampling VI. Capillary Electrophoresis and Electrokinetic Effects VII. Direct Sampling Combined with Mierofluidic Devices VIII. Microfluidics on Microchip Devices IX. Polymers X. Channel Geometry XI. Fabrication Techniques XII. Detection XIII. Biology XIV. Biological Application and Detection References Recent: Trends in Proteome Analysis Pier Giorgio Righetti, Annalisa Castagna, and Mahmoud Hamdan I. Introduction II. A Glimpse at Modem Technologies III. Some Basic Methodologies Pertaining to 2-D Page IV. Solubilization Cocktail V. Sample Reduction and Alkylation: When, Why, and How? VI. Mass Spectrometry and the Proteome: An Indissoluble Connubium VII. Informatics and Proteome: Interrogating Databases VIII. Quantitative Proteomics IX. Pre Fractionation in Proteome Analysis X. Conclusions: Quo Vadis, Proteome? References 7. Improving Our Understanding of Reversed Phase Separations for the 21st Century Patrick D. McDonald I. Introduction II. Background, III. Curious Incidents IV. Interdisciplinary Ideas and Inferences V. Conclusions References 8. Clinical Applications of High Performance Affinity Chromatography David S. Hage I. Introduction II. Traditional Methods III. Chiral Separations IV. Chromatographic Immunoassays.