Microfluidic Devices for Biomedical Applications

Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications.The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis.Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries. - Discusses the fundamentals of microfluidics or lab-on-a-chip (LOC) and explores in detail a wide range of medical applications - Considers materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies - Considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering

Contributor contact details Woodhead Publishing Series in Biomaterials About the editors Preface Part I: Fundamentals of microfluidic technologies for biomedical applications Chapter 1: Materials and methods for the microfabrication of microfluidic biomedical devices Abstract: 1.1 Introduction 1.2 Microfabrication methods 1.3 Materials for biomedical devices 1.4 Polymers 1.5 Conclusion and future trends 1.7 Appendix: acronyms Chapter 2: Surface coatings for microfluidic-based biomedical devices Abstract: 2.1 Introduction 2.2 Covalent immobilization strategies: polymer devices 2.3 Covalent immobilization strategies: glass devices 2.4 Adsorption strategies 2.5 Other strategies utilizing surface treatments 2.6 Examples of applications 2.7 Conclusion and future trends 2.8 Sources of further information and advice Chapter 3: Actuation mechanisms for microfluidic biomedical devices Abstract: 3.1 Introduction 3.2 Electrokinetics 3.3 Acoustics 3.4 Limitations and future trends Chapter 4: Digital microfluidics technologies for biomedical devices Abstract: 4.1 Introduction 4.2 On-chip microdrop motion techniques 4.3 Sensing techniques 4.4 Future trends 4.5 Conclusion Part II: Applications of microfluidic devices for drug delivery and discovery Chapter 5: Controlled drug delivery using microfluidic devices Abstract: 5.1 Introduction 5.2 Microreservoir-based drug delivery systems 5.3 Micro/nanofluidics-based drug delivery systems 5.4 Conclusion 5.5 Future trends Chapter 6: Microneedles for drug delivery and monitoring Abstract: 6.1 Introduction 6.2 Fabrication of microneedles (MNs) 6.3 MN design parameters and structure 6.4 Strategies for MN-based drug delivery 6.5 MN-mediated monitoring using skin interstitial fluid (ISF) and blood samples 6.6 Future trends 6.7 Conclusion Chapter 7: Microfluidic devices for drug discovery and analysis Abstract: 7.1 Introduction 7.2 Microfluidics for drug discovery 7.3 Microfluidics for drug analysis and diagnostic applications 7.4 Conclusion and future trends 7.5 Sources of further information and advice Part III: Applications of microfluidic devices for cellular analysis and tissue engineering Chapter 8: Microfluidic devices for cell manipulation Abstract: 8.1 Introduction 8.2 Microenvironment on cell integrity 8.3 Microscale fluid dynamics 8.4 Manipulation technologies 8.5 Manipulation of cancer cells in microfluidic systems 8.6 Conclusion and future trends 8.7 Sources of further information and advice Chapter 9: Microfluidic devices for single-cell trapping and automated micro-robotic injection Abstract: 9.1 Introduction 9.2 Device design and microfabrication 9.3 Experimental results and discussion 9.4 Conclusion 9.5 Acknowledgements Chapter 10: Microfluidic devices for developing tissue scaffolds Abstract: 10.1 Introduction 10.2 Key issues and technical challenges for successful tissue engineering 10.3 Microfluidic device platforms 10.4 Conclusion and future trends Chapter 11: Microfluidic devices for stem cell analysis Abstract: 11.1 Introduction 11.2 Technologies used in stem cell analysis 11.3 Examples of microfluidic platform for stem cell analysis: stem cell culture platform - mimicking in vivo culture conditions in vitro 11.4 Examples of microfluidic platform for stem cell analysis: single stem cell analysis 11.5 Microdevices for label-free and non-invasive monitoring of stem cell differentiation 11.6 Microfluidics stem cell separation technology 11.7 Conclusion and future trends Part IV: Applications of microfluidic devices in diagnostic sensing Chapter 12: Development of immunoassays for protein analysis on nanobioarray chips Abstract: 12.1 Introduction 12.2 Technologies 12.3 Immobilization chemistry 12.4 Detection methods 12.5 Applications 12.6 Conclusion and future trends Chapter 13: Integrated microfluidic systems for genetic analysis Abstract: 13.1 Introduction 13.2 Integrated microfluidic systems 13.3 Development of integrated microdevices 13.