Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip

Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip: Principles and Applications provides chemists, biophysicists, engineers, life scientists, biotechnologists, and pharmaceutical scientists with the principles behind the design, manufacture, and testing of life sciences microfluidic systems. This book serves as a reference for technologies and applications in multidisciplinary areas, with an emphasis on quickly developing or new emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology. The book offers practical guidance on how to design, analyze, fabricate, and test microfluidic devices and systems for a wide variety of applications including separations, disease detection, cellular analysis, DNA analysis, proteomics, and drug delivery. Calculations, solved problems, data tables, and design rules are provided to help researchers understand microfluidic basic theory and principles and apply this knowledge to their own unique designs. Recent advances in microfluidics and microsystems for life sciences are impacting chemistry, biophysics, molecular, cell biology, and medicine for applications that include DNA analysis, drug discovery, disease research, and biofluid and environmental monitoring.

Part I: Fundamentals of microfluidic/nanofluidic lab-on-a-chip1. Micromachining Methods2. Micromachining of silicon, glass devices3. Micromachining of polymers4. Fabrication of paper-based microfluidic devices5. Biocompatibility and surface modifications6. Systems interconnect and world-to-chip interface7. Microfluidic Flow control Part II: New Emerging Techniques8. Droplet microfluidics9. Closed-channel Droplet generation10. Open-format Digital microfluidics11. Nanofluidics12. Nanofabrication13. Characteristics of nanofluidics Part III: Applications14. Applications to Cell Biology15. Manipulation of cells 16. Cellular measurements and analysis17. Single-cell analysis18. 3D cell culture19. Organs-on-a-chip20. Applications to Nuclei Aids Analysis21. Nucleic acids extraction and purification22. Nucleic acids amplification23. Forensic DNA typing24. Microarray DNA chips25. Applications to proteins analysis26. Immunoassay27. Protein separation28. Enzymatic analysis29. Proteomics30. Applications to global health31. Low-cost Paper-based diagnosis devices32. Field diagnosis33. Other applications43. Environmental analysis44. Forensic analysis45. Small ions and molecules analysis46. Microreacter47. Perspective48. Instrument/device commercialization