Cell Culture Bioprocess Engineering, Second Edition

This book is the culmination of three decades of accumulated experience in teaching biotechnology professionals. It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive chapters. Practicality, including technological advances and best practices, is emphasized. This second edition consists of major updates to all relevant topics contained within this work. The previous edition has been successfully used in training courses on cell culture bioprocessing over the past seven years. The format of the book is well-suited to fast-paced learning, such as is found in the intensive short course, since the key take-home messages are prominently highlighted in panels. The book is also well-suited to act as a reference guide for experienced industrial practitioners of mammalian cell cultivation for the production of biologics.

Wei-Shou Hu is a Distinguished McKnight University Professor in the Department of Chemical Engineering and Materials Science at the University of Minnesota. His education and research efforts focus on bioprocess engineering and systems biotechnology. He is the author of Engineering Principles in Biotechnology and co-author of the textbook Bioseparations. For over three decades, he has been an active member in the cell culture bioprocess community. He is the founder of both a popular training course in cell culture bioprocess technology and an important cell technology conference that served as an important forum for cell technology advancement.

Preface; 1. Overview of Cell Culture Processes; 2. Cell Biology for Bioprocessing; 3. Cell Physiology; 4. Product Quality and Process; 5. Stoichiometry and the Kinetics of Cell Cultivation; 6. Cell Line Development; 7. Medium Design for Cell Culture Processing; 8. Cell Culture Bioreactors; 9. Fed-Batch Culture Processes; 10. Continuous Cell Culture Processes; 11. Oxygen Transfer in Cell Culture Bioreactors; 12. Scaling Up and Scaling Down for Cell Culture Bioreactors; Appendices: Cell Culture Process Data Analysis